Vol. 296, Issue 3, 1023-1034, March 2001

Lobeline Inhibits the Neurochemical and Behavioral Effects of Amphetamine

Dennis K. Miller, Peter A. Crooks, Lihong Teng, Jeffrey M. Witkin, Patrik Munzar, Steven R. Goldberg, Jane B. Acri and Linda P. Dwoskin

College of Pharmacy, University of Kentucky, Lexington, Kentucky (D.K.M., P.A.C., L.T., L.P.D.); and Behavioral Neuroscience Branch, National Institute on Drug Abuse Addiction Research Center, Baltimore, Maryland (J.M.W., P.M., S.R.G., J.B.A.)

Lobeline interacts with the dopamine transporter and vesicular monoamine transporter, presynaptic proteins involved in dopamine storage and release. This study used rodent models to assess lobeline-induced inhibition of the neurochemical and behavioral effects of amphetamine. Rat striatal slices were preloaded with [³H]dopamine and superfused with lobeline for 30 min, and then with d-amphetamine (0.03-3.00 µM) plus lobeline for 60 min. As predicted, lobeline (1-3 µM) intrinsically increased ³H overflow but did not inhibit d-amphetamine-evoked ³H overflow. Consequently, the effect of lobeline on d-amphetamine-evoked endogenous dopamine and dihydroxyphenylacetic acid overflow was assessed. Lobeline (0.1-1 µM) inhibited d-amphetamine (1 µM)-evoked dopamine overflow but did not inhibit electrically evoked ³H overflow, indicating a selective inhibition of this effect of d-amphetamine. To determine whether the in vitro results translated into in vivo inhibition, the effect of lobeline (0.3-10.0 mg/kg) pretreatment on d-amphetamine (0.1-1.0 mg/kg)-induced hyperactivity in rats and on d-methamphetamine (0.1-3.0 mg/kg)-induced hyperactivity in mice was determined. Doses of lobeline that produced no effect alone attenuated the stimulant-induced hyperactivity. Lobeline also attenuated the discriminative stimulus properties of d-methamphetamine in rats. Acute, intermittent, or continuous in vivo administration of lobeline (1-30 mg/kg) did not deplete striatal dopamine content. Thus, lobeline inhibits amphetamine-induced neurochemical and behavioral effects, and is not toxic to dopamine neurons. These results support the hypothesis that lobeline redistributes dopamine pools within the presynaptic terminal, reducing pools available for amphetamine-induced release. Collectively, the results support a role for lobeline as a potential pharmacotherapy for psychostimulant abuse.