JPET Introducing ALZET?ew Model 2006 Pump

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Köfalvi, A.
Right arrow Articles by Vizi, E. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Köfalvi, A.
Right arrow Articles by Vizi, E. S.

Vol. 295, Issue 2, 453-462, November 2000

Long-Lasting Facilitation of 4-Amino-n-[2,3-3H]butyric Acid ([3H]GABA) Release from Rat Hippocampal Slices by Nicotinic Receptor Activation1

Attila Köfalvi, Beáta Sperlágh, Tibor Zelles and E. Sylvester Vizi

Department of Pharmacology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary

In this study we explored the effect of the stimulation of nicotinic acetylcholine receptors located on interneurons by measuring 4-amino-n-[2,3-3H]butyric acid ([3H]GABA) release and monitoring [Ca 2+]i in superfused hippocampal slices. In the presence of 6-cyano-7-nitroquinoxaline-2,3-dione, (±)-2-amino-5-phosphonopentanoic acid, and atropine, i.e., under the blockade of N-methyl-D-aspartate and non-N-methyl-D-aspartate glutamate and muscarinic receptors, nicotine did not alter the spontaneous outflow of [3H]GABA, but significantly increased the stimulation-evoked [3H]GABA efflux. This effect of nicotine depended on the time interval between nicotine treatment and electrical stimulus, the concentration of nicotine (1-100 µM), and the parameters of electrical depolarization. Acetylcholine (0.03-3 mM), and the alpha 7 subtype-selective agonist choline (0.1-10 mM), also potentiated stimulus-evoked release of [3H]GABA, whereas 1,1-dimethyl-4-phenilpiperazinium iodide failed to increase the tritium outflow significantly. The effect of nicotine treatment was prevented by tetrodotoxin (1 µM) and by the nicotinic acetylcholine receptor antagonist mecamylamine (10 µM), and the alpha 7 subtype-selective antagonists alpha -bungarotoxin (100 nM) and methyllycaconitine (10 nM), whereas dihidro-beta -erythroidine (20 nM) was without effect. Perfusion of 100 µM nicotine caused a [Ca2+]i transient in about one-third of the tested interneurons; however, the response to subsequent electrical stimulation remained unchanged. Inhibition of the GABA transporter system by nipecotic acid (1 mM) or by decreasing the bath temperature to 12°C abolished completely the effect of nicotine to potentiate the stimulation-evoked release of GABA. These findings indicate that the activation of alpha 7-type nicotinic receptors of hippocampal interneurons results in a long-lasting ability of these cells to respond to depolarization with an increased release of GABA mediated by the transporter system.

1 This work was supported by a Philip Morris research grant and by the grants of Hungarian Research Foundation (OTKA), and the Hungarian Medical Research Council (ETT). Some information contained in this article was presented in preliminary form at the 29th Annual Meeting of Society for Neuroscience in Miami Beach, Florida (Sperlágh et al., 1999).

0022-3565/00/2952-0453$03.00/0
THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
Copyright © 2000 by The American Society for Pharmacology and Experimental Therapeutics


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Satta, E. Maloku, A. Zhubi, F. Pibiri, M. Hajos, E. Costa, and A. Guidotti
From the Cover: Nicotine decreases DNA methyltransferase 1 expression and glutamic acid decarboxylase 67 promoter methylation in GABAergic interneurons
PNAS, October 21, 2008; 105(42): 16356 - 16361.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
B. Lendvai and E. S. Vizi
Nonsynaptic Chemical Transmission Through Nicotinic Acetylcholine Receptors
Physiol Rev, April 1, 2008; 88(2): 333 - 349.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
J. Barik and S. Wonnacott
Indirect Modulation by {alpha}7 Nicotinic Acetylcholine Receptors of Noradrenaline Release in Rat Hippocampal Slices: Interaction with Glutamate and GABA Systems and Effect of Nicotine Withdrawal
Mol. Pharmacol., February 1, 2006; 69(2): 618 - 628.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
R. S. Hurst, M. Hajos, M. Raggenbass, T. M. Wall, N. R. Higdon, J. A. Lawson, K. L. Rutherford-Root, M. B. Berkenpas, W. E. Hoffmann, D. W. Piotrowski, et al.
A Novel Positive Allosteric Modulator of the {alpha}7 Neuronal Nicotinic Acetylcholine Receptor: In Vitro and In Vivo Characterization
J. Neurosci., April 27, 2005; 25(17): 4396 - 4405.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. Kofalvi, R. J. Rodrigues, C. Ledent, K. Mackie, E. S. Vizi, R. A. Cunha, and B. Sperlagh
Involvement of Cannabinoid Receptors in the Regulation of Neurotransmitter Release in the Rodent Striatum: A Combined Immunochemical and Pharmacological Analysis
J. Neurosci., March 16, 2005; 25(11): 2874 - 2884.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
M. Hajos, R. S. Hurst, W. E. Hoffmann, M. Krause, T. M. Wall, N. R. Higdon, and V. E. Groppi
The Selective {alpha}7 Nicotinic Acetylcholine Receptor Agonist PNU-282987 [N-[(3R)-1-Azabicyclo[2.2.2]oct-3-yl]-4-chlorobenzamide Hydrochloride] Enhances GABAergic Synaptic Activity in Brain Slices and Restores Auditory Gating Deficits in Anesthetized Rats
J. Pharmacol. Exp. Ther., March 1, 2005; 312(3): 1213 - 1222.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
M.-L. Si and T. J.F. Lee
{alpha}7-Nicotinic Acetylcholine Receptors on Cerebral Perivascular Sympathetic Nerves Mediate Choline-Induced Nitrergic Neurogenic Vasodilation
Circ. Res., July 12, 2002; 91(1): 62 - 69.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
All ASPET Journals Molecular Pharmacology Pharmacological Reviews
Molecular Interventions Drug Metabolism and Disposition

Copyright © 2000 by the American Society for Pharmacology and Experimental Therapeutics.