Vol. 282, Issue 1, 101-107, 1997

Analysis of Eicosanoid Mediation of the Renal Functional Effects of Hyperchloremia^1,2

Bardia Askari, Caroline P. Bell-Quilley³, David Fulton, John Quilley and John C. McGiff

Department of Pharmacology, New York Medical College, Valhalla, New York

Depression of GFR and antinatriuresis in response to high chloride has been linked to a cyclooxygenase (COX)-dependent mechanism involving thromboxane A₂ (TxA₂) and prostaglandin endoperoxide (PGH₂), because inhibition of COX prevented the fall in GFR and antinatriuresis produced by hyperchloremia. However, hyperchloremia did not increase, but unexpectedly decreased, renal prostaglandin and TxA₂ efflux (). To resolve questions regarding the role of eicosanoids in mediating the renal functional effects of high chloride (117 mM), by stimulating either TxA₂ synthesis or TxA₂/PGH₂ receptors, we compared the ability of indomethacin to block high-chloride effects in the rat isolated kidney with that of BMS 180291 and SQ 29548, antagonists of the TxA₂/PGH₂ receptor. These antagonists differ in terms of their selectivity and their capacity to inhibit isoforms of the TxA₂/PGH₂ receptor. Indomethacin and SQ 29548 had identical actions, preventing the decrease of GFR and antinatriuresis evoked by hyperchloremia, e.g., sodium excretion rate in the SQ 29548 and indomethacin groups increased to 7.2 ± 1.3 and 7.1 ± 1.2 µEq/min, respectively, compared with 2.6 ± 0.7 µEq/min in the control group. In contrast, neither BMS 180291 nor the TxA₂ synthase inhibitors, OKY 046 and CGS 13080, modified the negative effects of high chloride on GFR or sodium excretion. These results argue against either TxA₂ or PGH₂ acting as mediator of the effects of high chloride on renal function and suggest a product of COX activity such as a 20-HETE analog of prostaglandin endoperoxide. Evidence to support this proposal was obtained: 1) Hyperchloremia increased 20-HETE release from the rat kidney by 2-fold when compared with low-chloride conditions of renal perfusion. 2) The renal vasoconstrictor action of 20-HETE was shown to be dependent on COX activity and to be antagonized by blockade of the TxA₂/PGH₂ receptor.